HCG 5000

What it is

• Chemical Name: Human Chorionic Gonadotropin (hCG)
• Biological Source: Recombinant protein Molecular
• Weight: α-subunit: ~14.5 kDa; β-subunit: ~22.2 kDa
• Form: Sterile lyophilized powder
• Activity: 5,000 IU per vial
• Purity: ≥98% by SDS-PAGE and HPLC
• Solubility: Soluble in sterile water or phosphate buffered saline (PBS)
• Endotoxin Level: <1.0 EU per μg protein

Supplied as a lyophilized powder and sealed against light and moisture — shelf-stable, no cold chain required. Every lot is assayed by an independent laboratory and the certificate is published before the compound is listed. Strictly for in-vitro laboratory research.

Research applications

• Hormone receptor binding studies and affinity assays
• Cell culture supplementation for in vitro research
• Immunoassay development and calibration
• Glycoprotein hormone structure-function analysis
• Reference standard for analytical method validation
• Comparative endocrinology research
• Certificate of Analysis (CoA) provided with each lot
• SDS-PAGE gel confirming protein purity and molecular weight
• HPLC analysis for compositional verification
• Biological activity assay (IU determination)
• Endotoxin testing via LAL assay
• Sterility testing performed on final product
• Manufactured under GMP-compliant conditions

Storage & handling

• Storage (Lyophilized): Store at -20°C in original sealed vial. Protect from light and moisture.
• Storage (Reconstituted): Store at 2-8°C for up to 7 days. For extended storage, prepare aliquots and store at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
• Reconstitution: Reconstitute with sterile water, bacteriostatic water, or sterile PBS. Use aseptic technique. Allow to dissolve completely before use.
• Stability: Lyophilized product stable for 36 months when stored at -20°C as directed.
• Handling: Wear appropriate personal protective equipment. Handle according to institutional biosafety protocols for biological materials.

Research notes

Protein Structure: hCG is a heterodimeric glycoprotein hormone consisting of an α-subunit (shared with FSH, LH, and TSH) and a unique β-subunit.

Reconstitution Recommendations: For optimal stability, reconstitute at a concentration of 100-500 IU/mL. Higher concentrations may require the addition of carrier protein (e.g., 0.1% BSA) to prevent surface adsorption.

Activity Measurement: Biological activity is determined by in vitro bioassay and calibrated against WHO International Standard.

Classification: Analytical Standard / Research Use Only (RUO) biological material.

Regulatory Status: This material is not intended for diagnostic, drug, household, agricultural, cosmetic, or veterinary applications. Purchaser assumes full responsibility for compliance with all applicable federal, state, and local regulations governing the use and handling of biological research materials.

Specifications

• Form: Lyophilized powder
• Purity: ≥99% (HPLC verified)
• Presentation: 5000 IU
• Storage: Store lyophilized at −20°C; protect from light.